Journal article
A scalable, spin-free approach to generate enhanced induced pluripotent stem cell–derived natural killer cells for cancer immunotherapy
GR Rossi, J Sun, CY Lin, JKM Wong, L Alim, PY Lam, K Khosrotehrani, E Wolvetang, SW Cheetham, EB Derrick, A Amoako, C Lehner, AJ Brooks, PA Beavis, F Souza-Fonseca-Guimaraes
Immunology and Cell Biology | WILEY | Published : 2024
DOI: 10.1111/imcb.12820
Open access
Abstract
Natural killer (NK) cells play a vital role in innate immunity and show great promise in cancer immunotherapy. Traditional sources of NK cells, such as the peripheral blood, are limited by availability and donor variability. In addition, in vitro expansion can lead to functional exhaustion and gene editing challenges. This study aimed to harness induced pluripotent stem cell (iPSC) technology to provide a consistent and scalable source of NK cells, overcoming the limitations of traditional sources and enhancing the potential for cancer immunotherapy applications. We developed human placental–derived iPSC lines using reprogramming techniques. Subsequently, an optimized two-step differentiatio..
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Grants
Awarded by Australian Government
Funding Acknowledgements
We thank all members of the Guimaraes laboratory for their critical comments and advice. We also thank the TRI flow cytometry core for their support. This research was carried out at the Translational Research Institute, Woolloongabba, QLD, Australia. The Translational Research Institute is supported by grants from the Australian and Queensland Governments. The authors acknowledge the facilities and scientific and technical assistance provided by BASE at the University of Queensland. BASE was supported by the Therapeutic Innovation Australia (TIA). The TIA was supported by the Australian Government through the National Collaborative Research Infrastructure Strategy (NCRIS) program. We acknowledge the contribution of the South Australian Genomics Centre (SAGC) in the generation of data used in this publication. The SAGC is supported by its founding partner institutes and by Bioplatforms Australia as part of the Australian Government's National Collaborative Research Infrastructure Strategy (NCRIS). Open access publishing facilitated by The University of Queensland, as part of the Wiley - The University of Queensland agreement via the Council of Australian University Librarians.